Nei nitrogen yemvura ichishandiswa mune clyopreservation?
1. Nei uchishandisa mvura nitrogen sefairi?
1. Nekuti tembiricha ye mvura nitrogen Ichochocho chakanyanya kuderera, asi chimiro chayo chinyoro-nyoro, uye zvakaoma kune mvura nitrogen kuti vashandise zvemakemikari, saka zvinowanzoshandiswa sefairi.
2. Mvura nitrogen Vaporizy yekubvisa kupisa, kudzikisa tembiricha, uye inogona kushandiswa sefiriji.
3. Kazhinji, Ammonia inoshandiswa sefiriji uye mvura seyakawana.
4. VaAmonia gasi rinozorwazve neVanyori kuti vave Amonia mvura, uyezve Amonia mvura inopinda muvapi evapuru kuti vaburitse kunze kuti vazadzise chinangwa chefiriji, nekudaro vachiumba kusiyana kwakasiyana kwekushandiswa kwefiriji kutenderera.
5. Nitrogen inogona kushandiswa sefiriji mu "Cryogenic" mamiriro, izvo, zviri pedyo nemadhigirii madhigirii (-273),
6. Mukurapa, mvura nitrogen inowanzo shandiswa sechifare kuti iite mabasa pasi peCryoanthesia.
7. Mune yakakwirira-tech munda, mvura nitrogen inowanzoshandiswa kugadzira yakaderera-tembiricha nharaunda. Semuenzaniso, mamwe mashura ekusakura anowana mashura anowana zvivakwa pamhepo dzinodziya mushure mekubatwa nedombo nitrogen.
8 Iyo yakaderera-tembiricha ichipwanya matairi, kuchengetwa kwenzvimbo muzvipatara, etc. zvese shandisa mvura nitrogen seyakawoneka.
2. Mvura Nitrogen chengetedza masero sei?
Iyo inowanzo shandiswa nzira yeCell Clyopreserders ndiyo yemvura nitrogen Cryopreserrester nzira, iyo inonyanya kutora nzira inononoka kutonhora ine huwandu hwakakodzera hwemumiriri wekudzivirira kuti udzivise maseru.
Ongorora: Kana masero ari kuomesa zvakananga pasina kuwedzerwa chero mumiririri anodzivirira, mvura mukati uye kunze kwemasero inokurumidza kuumba echando makristuru, ayo anozadzisa nhevedzano yekuita zvakaipa. Semuenzaniso, kushomeka kwemasero anowedzera kusungwa kwemunharaunda, anoshandura kukosha kwePF, uye anoshongedza mamwe mapuroteni nekuda kwezvikonzero zvisizvo, zvichikonzera chimiro chemukati chechitokisi chichakanganisika. Inokonzera kukuvara, mitochondrial kuzvimba, kurasikirwa kwebasa, uye kukanganisa kwesimba metabolism. Iyo Lipoprotein yakaoma pamamita yemasero inoparadzaniswa nyore nyore, zvichikonzera shanduko mukuparara kwesero membrane uye kurasikirwa kwesero zvirimo. Kana akawanda echando echando akaumbwa mumasero, sezvo tembiricha yemahara inodzikira, iyo vhoriyamu yemakristuru echando ichakura, ichikonzeresa kukuvara kweiyo spatial kugadziriswa kweNuclear DNA, zvichikonzera rufu rweCell.
Iyo yekupedzisira uye yekungwara kupisa kwakanyudzwa neyekudya kwechitoro cheNitrogen nekuonana nechikafu chinokonzera chikafu chisingaperi. Liquid nitrogen inobviswa kubva mumudziyo, kamwe kamwe shanduko kune yakajairika tembiricha uye kumanikidzwa, uye kushandura kubva mumvura kusvika kune gireous State. Munguva iyi chikamu cheshanduko maitiro, mvura nitrogen mamota uye inoputika pa -195. Kana -19.8 Kana tembiricha kana tembiricha ° C Underping International Pressure, (KGI? K) Kupisa kunogona kusvika 383 kj / kg.
Mukuita kwekudya kwekudya, nekuti huwandu hwakawanda hwekupisa unobviswa pakarepo, kupisa kwechikafu kwakadzima kubva kunze kune mukati kusimuka. Liquid nitrogen yekukurumidza-kusununguka tekinoroji inoshandisa mvura nitrogen seya inotonhora sosi, iyo haina kukuvadza nharaunda. Kuenzaniswa neFirizi yeChinyakare mupaririji, inogona kuwana tembiricha yakadzika uye yakakwira inotonhorera chiyero. Liquid nitrogen inokurumidza-kusununguka tekinoroji ine nekukurumidza kumhanya, nguva pfupi, uye chikafu chine kunaka, kuchengetedzwa kwakanyanya uye kusvibiswa-kusvibiswa.
Liquid nitrogen-freezing tekinoroji yakashandiswa zvakanyanya mukukurumidza-kusununguka kwezvigadzirwa zvemumvura senge shrimp, Whitebait, Biologicit, Biological Crub, naAhasione. Zvidzidzo zvakaratidza kuti shrimp kurapwa neyemvura nitrogen nekukurumidza-kusununguka tekinoroji inogona kuchengetedza yakanyanya kutsva, ruvara nekuvira. Kwete izvozvo chete, mamwe mabhakitiriya anogona kuurayirwawo kana kumira kubereka pakudzora kwakadzika kuti uwane huwandu hwakakwira huchiwana.
Cricureservation: Liquid nitrogen inogona kushandiswa kune clyopreservreservreserders yezvinhu zvakasiyana-siyana zvebonde, senge maseru, etc. izvi samples dzinochengetwa kwenguva yakareba pakudziya nekudzoreredzwa kune yavo yekutanga mamiriro kana zvichidikanwa. Liquid nitrogen clopreservation ndiyo nzira inowanzo shandiswa nzira, inowanzoshandiswa muBiomemical kutsvagisa, kurima, yemhuka uye mamwe minda.
Tsika yeCell: Liquid nitrogen inogona zvakare kushandiswa kune tsika tsika. Munguva yemutero, mvura nitrogen inogona kushandiswa kuchengetedza masero ekutevera ekuedza kwekuedza. Liquid nitrogen inogona zvakare kushandiswa kusunungura masero kuti achengetedze quility yavo uye yebhizimusi zvivakwa.
Kuchengetedza cell: Iyo yakadzika tembiricha yemvura nitrogen inogona kuchengetedza kugadzikana uye kuvimbika kwemasero, uku uchidzivirira kuchembera uye kufa. Naizvozvo, nitrogen iri inoshandiswa zvakanyanya mukuchengetedza cell. Masero akachengetedzwa mumvura nitrogen inogona kupora nekukurumidza kana ichidikanwa uye inoshandiswa kune dzakasiyana siyana dzekuedza.
Iko kushandiswa kweGiredhi-giredhi mvura nitrogen nitrogen Ice cream, mvura nitrogen mabhisiketi uye anesthesia mukurapa zvakare anodawo yakakwirira-kuchena kwemvura nitrogen. Mamwe maindasitiri akadai seindasitiri yemakemikari, zvemagetsi, metallurgy, etc. ine zvakasiyana zvinodiwa zvekuchena kwemvura nitrogen yemvura.
