Vim li cas thiaj ua kua Nitrogen siv hauv cryopreservation?
1. Vim li cas siv cov kua nitrogen raws li lub tub yees txias?
1. Vim hais tias qhov kub ntawm Ua kua nitrogen Nws tus kheej yog tsawg heev, tab sis nws qhov yog qhov nyuaj heev, thiab nws yog qhov nyuaj rau ua kua nitrogen rau yov cov tshuaj lom neeg, yog li nws feem ntau siv ua lub tub yees.
2. Ua kua nitrogen vaporizes nqus cua sov, qis dua qhov kub thiab txias, thiab tuaj yeem siv ua lub tub yees txias.
3. Feem ntau, ammonia yog siv ua tub yees thiab dej raws li kev nqus.
4. Ammonia cov roj yog txias los ntawm cov kmmorator los ua kua rau sab nraud kom haum rau kev hloov pauv tub yees.
5. Nitrogen tuaj yeem siv ua lub tub yees hauv "cryogenic" tej yam kev mob, thiab feem ntau siv nyob rau hauv chaw soj ntsuam kom kawm supercondies.
6. Hauv cov tshuaj, kua nitrogen feem ntau siv ua lub tub yees ua haujlwm ua haujlwm hauv qab teb cryloanesthesia.
7. Nyob rau hauv lub siab-tech teb, ua kua nitrogen feem ntau siv los tsim ib puag ncig kub tsawg-tsawg. Piv txwv li, qee cov khoom lag luam superconduction tsuas yog kom tau superconducting cov yam ntxwv ntawm qhov kub kub tom qab kho nrog ua kua nitrogen.
8. Qhov kub thiab txias nyob rau hauv lub siab ua kua ntawm cov kua nitrogen yog -196 degrees, uas tuaj yeem siv ua ib qho chaw txias ultra-tsawg. Qhov tsawg-kub crushing ntawm cov log tsheb, noob ntim khoom hauv tsev kho mob, thiab lwm yam siv ua kua nitrogen ua lub zog ua kua.
2. Yuav ua li cas ua kua nitrogen tiv thaiv hlwb?
Feem ntau cov txheej txheem siv rau cell canopreservation yog cov ua kua nitrogen kev kawm kom khov nrog tus nqi tiv thaiv kom khov rau cov qe khov.
NCO TSEG: Yog tias lub hlwb yog ncaj ncaj yam tsis muaj ib tus neeg tiv thaiv, cov dej sab hauv yuav ua cov dej khov sai sai, uas yuav ua rau muaj kev phiv tshuaj sai sai. Piv txwv li, lub cev qhuav dej ntawm lub hlwb nce cov khoom siv hluav taws xob hauv zos, thiab cov khoom siv pH vim tias cov qauv sab hauv uas muaj kev cuam tshuam. Ua rau muaj kev puas tsuaj, mitochondrial o, poob ntawm kev ua haujlwm, thiab kev ntxhov siab ntawm cov metabolism hauv lub zog. Lub lipoprotein txojs ntawm lub cell membrane kuj yooj yim rhuav tshem, ua rau cov kev hloov pauv ntawm cov cell membrane thiab poob ntawm cov ntsiab lus ntawm tes. Yog tias muaj cov dej khov ntau yog tsim nyob rau hauv lub hlwb, raws li lub khov kub txo qis, ua rau cov dej khov nab kuab yuav nthuav dav ntawm cov nuclear DNA, uas ua rau ntawm txoj kev tuag ntawm tes.
Cov latent thiab paub tab sib nqus cua sov nqus los ntawm cov khoom noj uas muaj kua hauv kev sib chwv nrog cov zaub mov ua rau zaub mov khov. Ua kua nitrogen yog laij tawm ntawm lub thawv, maj mam hloov mus rau qhov ntsuas kub qub thiab lub siab, thiab hloov pauv ntawm cov kua rau gaseous lub xeev. Lub sijhawm theem no hloov pauv, ua kua nitrogen boils thiab evapates ntawm -195.8 ℃ kom ua kom sov ntawm evaporation, thiab Latent Tshav Kub ntawm yog 199 KJ; Yog tias -195.8 Thaum lub qhov kub nce rau -20 ° C nyob rau hauv nitrogen ntawm cov cua sov (kg / kg / kg? k)) Cov cua sov tuaj yeem ncav cuag 383 KJ / kg.
Nyob rau hauv cov txheej txheem ntawm cov zaub mov khov, vim tias muaj cua sov ntau tam sim ntawd nyob rau hauv ib qho chaw so, qhov kub ntawm cov zaub mov tau txias nrawm ntawm sab nraud mus rau sab hauv kom khov. Ua kua nitrogen ceev thev naus laus zis siv cov tshuaj nitrogen ua kua dej ua kom txias, uas tsis muaj kev phom sij rau ib puag ncig. Piv nrog cov khoom siv kho tshuab ib txwm muaj, nws tuaj yeem ua tiav qis dua qhov ntsuas kub thiab siab dua txias. Ua kua nitrogen ceev thev naus laus zis ntau nrawm nrawm nrawm, thiab cov khoom noj muaj txiaj ntsig zoo, muaj kev nyab xeeb thiab muaj kuab paug.
Ua kua nitrogen ceev thev naus laus zis tau siv dav nyob rau hauv cov khoom lag luam ceev ntawm cov khoom lag luam uas tsis muaj mob, thiab pob hluav taws xob hauv nkoj, thiab tso hluav taws xob. Cov kev tshawb fawb tau pom tias cws kho los ntawm kua nitrogen ceev thev naus laus zis tuaj yeem ua kom lub siab tshiab, xim thiab saj. Tsis tsuas yog tias, qee cov kab mob kuj tseem yuav raug tua lossis nres kev ua tub luam ntawm qhov kub tsawg kom ua tiav qhov siab dua yuav tsum tau huv.
Cryopreservation: Ua kua nitrogen tuaj yeem siv rau cryopreservation ntawm ntau yam kev kuaj pom ntawm cov qauv ntev thiab rov qab rau lawv thawj lub xeev thaum xav tau. Ua kua Nitrogen Kev Tshawb Fawb Ua Si yog ib qho kev siv khoom siv ib txwm siv hauv kev tshawb fawb, ua liaj ua teb, tsiaj cov tsiaj thiab lwm thaj teb.
Cellure Kev lis kev cai: kua nitrogen tseem siv tau rau kev coj noj coj ua ntawm tes. Thaum lub sij hawm cell kab lis kev cai, ua kua nitrogen tuaj yeem siv los khaws cia cov haujlwm sim ua tom ntej. Ua kua nitrogen kuj tseem siv tau rau cov qe khov los khaws lawv cov peev xwm thiab cov khoom siv biological.
Cell cia: qhov kub tsawg ntawm cov kua nitrogen tuaj yeem tswj hwm qhov kev ruaj ntseg thiab kev sib daj sib deev ntawm cov kev laus thiab kev tuag. Yog li ntawd, ua kua nitrogen tau siv dav hauv cell cia. Cov hlwb khaws cia rau hauv cov kua nitrogen tuaj yeem rov qab tau sai sai thaum xav tau thiab siv rau ntau txoj kev sim.
Daim ntawv thov ntawm cov zaub mov-qib kua dej yog zoo li ua kua nitrogen biscuits thiab kua qaub ua kua rau hauv cov tshuaj kuj xav tau kev lom zem ua kua nitrogen. Lwm cov kev lag luam xws li kev lag luam tshuaj, hluav taws xob, metallurgy, thiab lwm yam yuav tsum muaj cov kev ua kom sib txawv ntawm cov kua nitrogen.
